Chromobody®-Technology |
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Chromobodies® are novel fluorescent nanoprobes that are extremely small and highly stable. In comparison to conventional antibodies Chromobodies® are suitable for real time analyses and visualization of endogenous cellular structures and processes in live cells. Thus Chromobodies® are the perfect reagents for cellular research including High-Content Analysis (HCA). |
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U2OS Cell Cycle Chromobody® ***now available*** |
ChromoTek's powerful U2OS Cell Cycle Chromobody® enables you to screen for compounds such as cancer drugs for effects on the cell cycle and toxicity in one experiment. You can apply this HCA assay in early drug development and validation as well as in basic research.
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Cell Cycle Chromobody® signal during the cell
cycle. In G1 the Chromobody® signal is
homogeneously distributed throughout the
nucleus and cytoplasm. During S phase it
accumulates in the nucleus and visualizes
the formation of replication foci. In G2 the
foci disappear and the cell divides (mitosis). |
Automated evaluation of Cell Cycle Chromobody® signal to identify cells in S phase (blue). Measurement of the cytoplasmic area was used to identify cells rounding up and entering mitosis (red). |
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for simultaneous read out of:
- cell size and morphology
- nuclear morpholgy
- progression of S phase (detailed detection of early-mid-late S phase)
- mitosis
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features:
- U2OS Cell Cycle Chromobody® to trace dynamic changes during the cell cycle in real time
- U2OS Cell Cycle Chromobody® to monitor the distribution of an endogenous cell cycle marker protein - in comparison to fluorescent fusion proteins no overexpression artifact or cytotoxic effects
- U2OS Cell Cycle Chromobody® does not affect function of the cell
- U2OS Cell Cycle Chromobody® is available as stable fluorescent U2OS cell lines ready to go for cellular screening
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coming soon:
- HeLa Cell Cycle Chromobody®
- Semi-stable Cell Cycle Chromobody® cell lines (including primary cells from mouse/human)
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request info and pricing
Product brochure (PDF)
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Only for research applications, not for diagnostic or therapeutic use. |
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