products GFP-Trap® RFP-Trap® Booster Chromobodies®   ***new*** Monoclonal antibodies Binding controls

products

RFP-Booster_Atto594

  code price size
  rba594 €250.00 100 µg
Description RFP-Trap® coupled to fluorescent dye ATTO 594

Relevance

Fluorescent fusion tags enable studying of protein localization and dynamics on cellular and molecular levels. Red fluorescent proteins (RFPs) are widely used as fluorescent tags. Commonly, a combination of red and green fusion proteins (GFPs) is employed to allow multicolor tracking and analysis of protein-protein interactions. However, the quantum yield and photostability of RFPs are often not sufficient for their efficient microscopic detection. This can be especially critical for Super Resolution Microscopy applications (e.g. 3D-SIM or STED). Furthermore, many cell biological methods such as HCl treatment for BrdU-detection, the EdU-Click-iT™ treatment or heat denaturation for FISH lead to disruption of RFP signal.
Use RFP-Booster_Atto594, a specific RFP-binding protein coupled to the fluorescent dye ATTO 594 (from ATTO-TEC), to brighten up and stabilize your RFP signal.

Specificity

RFP-Trap® efficiently detects and labels monomeric red fluorescent protein from Discosoma sp. such as mRFP, mCherry or mPlums.
Tested Applications and Recommended Dilutions
Immunofluorescence
 
RFP-Booster specifically labels RFP-fusion proteins.
HeLa cells expressing mRFP-PCNA, fixed and permeabilized. Specifical staining of early, mid and late replication foci with RFP-Booster_Atto594 (dilution 1/200, 1 h at RT).		  
 
 
Enhancement of RFP signal with RFP-Booster_Atto594.
Comparison of signal intensity of a HeLa cell line stably expressing a nuclear RFP-fusion protein before and after RFP-Booster treatment (1/200 dilution).		  
 
 

Improvement of RFP signal stability with RFP-Booster_Atto594. RFP fluorescence bleaches very quickly upon irradiation with high laser intensity. In contrast, fluorescent signal remains stable when enhanced with RFP-Booster_Atto594

  

Optimal dilutions/concentrations should be determined by the end user.
User Comments

“I tested the RFP-Booster for labeling Drosophila oocytes using the suggested protocol. The Booster was specific and had double the fluorescence intensity as the fixed mCherry control. Even more exciting was that the dye was wonderfully stable. We imaged 9000 frames and had very little (if any) bleaching.”
Dr. Timothy Weil, Oxford
Concentration 0.2 mg/ml
Storage instructions Shipped at RT. Store at 4°C, do not freeze. Protect from light.
 

Only for research applications, not for diagnostic or therapeutic use.